Galactose Oxidase of Polyporus circinatus: a Copper Enzyme
نویسندگان
چکیده
منابع مشابه
Galactose oxidase of Polyporus circinatus: a copper enzyme.
In 1959, Cooper et al. (1) described the isolation of an enzyme from Polyporus circinatus Fr. which catalyzed the oxidation of galactose by molecular oxygen. MTe have previously described a procedure for the purification of such an enzyme from culture filtrat,es and have shown the reaction product to be D-g&CtOhexodialdose, oxidation occurring at the C-6 rather than the C-l position (2). The pr...
متن کاملThe D-galactose oxidase of Polyporus circinatus.
The mold Polyporus circinatus produces an extracellular oxidase that catalyzes the oxidation of n-galactose (1). The reaction appears superficially to be similar to the oxidation of n-glucose catalyzed by notatin (2)) and can be followed by measuring the consumption of oxygen or the production of Hz02 (1). The enzyme is useful for the determination of galactose, employing the same peroxidase-ch...
متن کاملMechanistic studies on the single copper tyrosyl- radical containing enzyme galactose oxidase
Studies on the single Cu protein galactose oxidase (68kDa; 639 amino acids) from Fusarium NRRL 2903 are described. The Cu is coordinated in a square based pyramid by Tyr-272, Tyr-495 (axial), His-496, His-581 and H20 (the substrate binding site), and the enzyme functions as a 2-equivalent oxidase, 0, + H,O,, with oxidation of primary alcohol substrates RCH20H to RCHO. The active enzyme has a co...
متن کاملEnhanced enzyme properties by protein engineering of galactose oxidase
We have investigated a series of single and double mutants and a triple mutant comprising V494A, C383S and Y436H identified by directed evolution. The locations of the mutated residues are shown in Fig. 1. The most interesting effect is due to the C383S mutation that leads to a significant reduction in KM. Cys 383 lies in a pocket at the back of the active site behind the copper atom and theref...
متن کاملHeavily fluorinated carbohydrates as enzyme substrates: oxidation of tetrafluorinated galactose by galactose oxidase.
Galactose oxidase (GOase) was shown to oxidise several C2/C3 fluorinated galactose analogues. Interestingly, the enzyme was able to distinguish between the 2,3-tetrafluorinated galactose and its epimeric glucose analogue, and this represents the first reported biotransformation of a heavily fluorinated sugar.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)67966-4